Remember how last time I mentioned that nematodes could have their eggs frozen? This week I was able to thaw some strains. One of them was NP1579. They keep frozen strains in the freezer at -80 ˚C ( -112 ˚F), so cold that it can start to feel like a burning.
I've thawed other strains like NP1678 and NP1662, and I'll be using them to run a Western blot to figure out the location of particular proteins. The two strains contain genes (for the proteins) F11E6.1 and CPR-6 (C25B8.3) which're homologues to Glucosylceramidase and CATHEPSIN B in humans, respectively. Those're two proteins that are thought to be involved in mucolipidosis type IV (MLIV), but we don't know very much about them.
I'm still learning about the process and I've been doing plenty of reading.
Wishing you guys the best!
I've thawed other strains like NP1678 and NP1662, and I'll be using them to run a Western blot to figure out the location of particular proteins. The two strains contain genes (for the proteins) F11E6.1 and CPR-6 (C25B8.3) which're homologues to Glucosylceramidase and CATHEPSIN B in humans, respectively. Those're two proteins that are thought to be involved in mucolipidosis type IV (MLIV), but we don't know very much about them.
I'm still learning about the process and I've been doing plenty of reading.
Wishing you guys the best!
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